pwpi plasmid Search Results


blasticidin resistance genes  (Addgene inc)
93
Addgene inc blasticidin resistance genes
Blasticidin Resistance Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blasticidin resistance genes - by Bioz Stars, 2026-03
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fluorescent protein gfp  (Addgene inc)
95
Addgene inc fluorescent protein gfp
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Fluorescent Protein Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescent protein gfp/product/Addgene inc
Average 95 stars, based on 1 article reviews
fluorescent protein gfp - by Bioz Stars, 2026-03
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pwpi ires puro ak ace2 tmprss2  (Addgene inc)
93
Addgene inc pwpi ires puro ak ace2 tmprss2
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Pwpi Ires Puro Ak Ace2 Tmprss2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pwpi ires puro ak ace2 tmprss2 - by Bioz Stars, 2026-03
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human prkci vector  (Addgene inc)
90
Addgene inc human prkci vector
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Human Prkci Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human prkci vector - by Bioz Stars, 2026-03
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sonja best addgene plasmid  (Addgene inc)
93
Addgene inc sonja best addgene plasmid
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Sonja Best Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sonja best addgene plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
sonja best addgene plasmid - by Bioz Stars, 2026-03
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pwpi ires puro ak  (Addgene inc)
93
Addgene inc pwpi ires puro ak
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Pwpi Ires Puro Ak, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pwpi ires puro ak/product/Addgene inc
Average 93 stars, based on 1 article reviews
pwpi ires puro ak - by Bioz Stars, 2026-03
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human ace2  (Addgene inc)
93
Addgene inc human ace2
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Human Ace2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ace2/product/Addgene inc
Average 93 stars, based on 1 article reviews
human ace2 - by Bioz Stars, 2026-03
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pwpi hplk2wt neo  (Addgene inc)
90
Addgene inc pwpi hplk2wt neo
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Pwpi Hplk2wt Neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pwpi hplk2wt neo - by Bioz Stars, 2026-03
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reference pwpi ires bla ak tmprss2 plasmid  (Addgene inc)
93
Addgene inc reference pwpi ires bla ak tmprss2 plasmid
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Reference Pwpi Ires Bla Ak Tmprss2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reference pwpi ires bla ak tmprss2 plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
reference pwpi ires bla ak tmprss2 plasmid - by Bioz Stars, 2026-03
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lentivectorpwpi spbfgf  (Addgene inc)
92
Addgene inc lentivectorpwpi spbfgf
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Lentivectorpwpi Spbfgf, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentivectorpwpi spbfgf - by Bioz Stars, 2026-03
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pwpi ires puro ak tmprss2  (Addgene inc)
92
Addgene inc pwpi ires puro ak tmprss2
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Pwpi Ires Puro Ak Tmprss2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pwpi ires puro ak tmprss2/product/Addgene inc
Average 92 stars, based on 1 article reviews
pwpi ires puro ak tmprss2 - by Bioz Stars, 2026-03
92/100 stars
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insular cortex  (Addgene inc)
92
Addgene inc insular cortex
HIF-1α overexpression in MSCs. (A) pWPI <t>GFP</t> and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green <t>fluorescent</t> protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.
Insular Cortex, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/insular cortex/product/Addgene inc
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Image Search Results


HIF-1α overexpression in MSCs. (A) pWPI GFP and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green fluorescent protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.

Journal: Stem Cells and Development

Article Title: Mesenchymal Stem Cell Migration and Proliferation Are Mediated by Hypoxia-Inducible Factor-1α Upstream of Notch and SUMO Pathways

doi: 10.1089/scd.2016.0331

Figure Lengend Snippet: HIF-1α overexpression in MSCs. (A) pWPI GFP and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs ( gray ) relative to MSCs ( black ) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs ( gray ) relative to MSCs ( black ); α-tubulin was used as a protein loading control. ( E ) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. * P < 0.05, *** P < 0.001. GFP, green fluorescent protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.

Article Snippet: Briefly, cells were transduced with pWPI-green fluorescent protein (GFP) or pWPI-HIF-1α-GFP expression vectors ( http://addgene.org cat. #12254) daily for 3 days.

Techniques: Over Expression, Labeling, Gene Expression, Quantitative RT-PCR, Western Blot, Control, Confocal Microscopy, Real-time Polymerase Chain Reaction, Standard Deviation